Microdetermination of Urea Nitrogen in Blood with Diacetyl Monoxime-Glucuronolactone Reagent

Determination of Urea in Blood and Urine with Diacetyl Monoxime-glucuronolactone Reagent.

N UMEROUS STUDIES have been reported in the literature on the photometric determination of urea in biological fluids with diacetyl monoxime as the color-developing agent. The reaction is photosensitive, does not follow Beer’s law, and requires special precautions for reproducible results. In many proposed procedures, oxidants were added in the reaction mixture to destroy hydroxylamine which mig...

A new colorimetric method for the determination of urea nitrogen in blood with diacetyl monoxime-glucuronolactone-glucosaccharodilactone reagent.

In a previous paper,3) a colorimetric method for the determination of urea nitrogen in blood and urine was presented on the basis of a color reaction of urea with diacetyl monoxime (DAM) in phosphoric acid solution in the presence of D-glucuronolactone (GL), which served to produce a photo-stable color and eliminate the sigmoidal nature of calibration curve observed usually in the DAM method.4)...

A study of the reaction of urea with diacetyl monoxime and diacetyl.

The automated thiosemicarbazide-diacetyl monoxime method for plasma urea.

IN RECENT YEARS the automated diacetyl monoxime (DAM) reaction as adapted to the AutoAnalyzer has become the method of choice in many clinical laboratories for the determination of urea in biologic fluids. The method is based on the condensation of urea with diacetyl, the latter usually being liberated from the monoxime in the presence of one of various acid reagents (1, 2). The reaction, howev...

A note on urea estimation by diacetyl monoxime.

Rosenthal (1955) has described in great detail a method using diacetyl monoxime which is generally satisfactory for the routine estimation of urea in blood and urine except that complete colour development requires prolonged heating in boiling water. This disadvantage can be overcome by the use of a pressure cooker. The tubes of reaction mixture are immersed in a beaker filled with hot water to...